SPIB Immunoprecipitation using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate 4 µL with SPIB Polyclonal Antibody (Product # PA5-81265) and 15 µL of 50 % Protein G agarose. Primary antibody: SPIB Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 44 kDa.
Supplier Page from Thermo Fisher Scientific for SPIB Antibody